TOLERANCIA IN VITRO DE BIOPELÍCULAS POLIMICROBIANAS DE STAPHYLOCOCCUS HAEMOLYTICUS Y CANDIDA ALBICANS A LA ACTIVIDAD ANTIINFECCIOSA DE LA DALBAVANCINA EN COMBINACIÓN CON ANIDULAFUNGINA

Abstract

Infections caused by the formation of bacterial biofilms on invasive devices such as catheters and prostheses are a public health problem. The coagulase-negative bacteria Staphylococcus haemolyticus is a recurrent pathogen in these infections, as well as the yeast Candida albicans. The presence of both microorganisms and infections caused by catheters in these devices is common. These microorganisms grow by forming biofilms, which makes it difficult to treat their infections and hinders the patient's recovery. Dalbavancin is a semisynthetic lipo-glycopeptide class antibiotic used as an anti-infective agent to eliminate biofilms of largely gram-positive microorganisms. Anidulafungin is an echinocandin class antifungal drug with high efficacy against resistant yeast strains, and is a biofilm removal agent. OBJECTIVE: To evaluate the increase in resistance to anidulafungin and dalbavancin of Staphylococcus haemolyticus and Candida albicans when grown in polymicrobial biofilms compared to their growth in liquid cultures. METHODS: In vitro assays using polymicrobial biofilm growth in a CDC biofilm reactor (CBR). RESULTS: The minimum inhibitory concentration of anidulafungin against Candida albicans increased when it grew in biofilms, while the activity of dalbavancin against Staphylococcus haemolyticus biofilms did not present changes with respect to its action on planktonic forms. CONCLUSIONS: An increase in the tolerance of C. albicans to AFG was observed in polymicrobial biofilms, while the resistance of S. haemolyticus to DAL did not show significant changes in the same environment.